基于COⅡ基因的我国海域4个长蛸群体的遗传变异研究毕业设计_计算机程序设计_计算机设计辅导_计算机设计代做_java_安卓_小程序_springboot

摘要

采用PCR扩增技术对中国4个不同海域（山东青岛、浙江温州、浙江舟山、福建东山）的长蛸群体的COII基因片段进行了扩增和测序，获得了长度为560bp长度的同源序列，分析了不同海域群体内和群体间的遗传变异。结果表明，89个个体共有28个单倍型，其中青岛群体8个，舟山群体7个，温州群体6个，东山群体7个。通过序列比对，变异均匀地分布于序列各个区域，但密码子偏好不明显。总群体单倍型多样性指数（H）0.884±0.018，核苷酸多样性指数（Pi）0.10640±0.00656，平均核苷酸差异数（K）57.348，显示出一定的遗传多样性。采用MEGA3.1对4个海域的长蛸群体进行聚类分析，构建系统树，结果表明：青岛、舟山2个群体由于遗传距离较近首先聚为一支，而与东山群体遗传关系较远，与温州群体亲缘关系最远而最后聚类。Tajima’s D和Fu’s FS检验显示温州群体可能经历过种群扩张，而其它3个群体可能未经历过历史扩张事件。我国海域长蛸的这种遗传结构的形成原因还有待于进一步证实。通过本次研究，从线粒体DNA水平分析我国不同海域长蛸群体的遗传变异情况，期望为开展我国长蛸群体的资源评价、物种保护及分子标记育种提供基础资料。

关键词：长蛸；线粒体基因；COⅡ基因；遗传变异

Abstract

Fragments of COII gene were amplified and sequenced.in Octopus variabilis samples from 4 our different sea areas in China (Qingdao, Wenzhou, Zhoushan, Fujian) A 560bp aligned sequence were obtained and analyzed to detect genetic variation within and among the groups. The results showed that 89 individuals produce a total of 28 haplotypes, with eight in Qingdao group, seven in Zhoushan group, six in Wenzhou group, seven in Dongshan group. By sequence alignment, the variation is evenly distributed in the various regions of the sequence, but the codon bias is not obvious. The total population haplotype diversity index (H) is 0.884 ± 0.018, nucleotide diversity index (Pi) is 0.10640 ± .00656, the average number of nucleotide differences (K) is 57.348, showing a certain degree of genetic diversity. A phylogenetic tree among the 4 Octopus variabilis by adopting MEGA3.1 software showed that: the genetic distance among Qingdao and Zhoushan group was minimum. the genetic distance between those two and Dongshan or Wenzhou group are much big. Wenzhou groups may have experienced a population expansion, while the other three groups may not have experienced a historical expansion events. The phylogenetic tree reflects the genetic differences between the geographical distribution of these groups. Through this study, the level of mitochondrial DNA analysis of genetic variation in different waters Octopus variabilis groups expect to carry out the group of China’s Octopus variabilis resource assessment, protection of species and molecular marker breeding genetic data.

Key words：Octopus variabilis; mitochondrial genes; COII gene; Genetic variation

摘要………………………………………………………… I

Abstract…………………………………………………….. II

引言………………………………………………………… 1

1 材料与方法………………………………………………….. 2

1.1实验材料………………………………………………….. 2

1.2实验药品与试剂…………………………………………….. 2

1.2.1 实验所用的基本试剂……………………………………….. 2

1.2.2 自配试剂………………………………………………… 2

1.2.3 琼脂糖凝胶板的制备……………………………………….. 3

1.3 主要仪器与设备…………………………………………….. 3

1.4 实验耗材………………………………………………….. 4