摘要

高效液相色谱-质谱(HPLC-MS)联用技术是近几年来发展起来的一项新的分离分析技术。它将应用范围极广的分离方法——液相色谱法与灵敏、专属、能提供结构信息的质谱法结合起来，成为一种重要的现代分离分析技术。液质联用为生产和科研提供了许多有价值的数据，解决了许多在此之前难以解决的分析问题。本文主要探索液质联用的条件，并运用LC-MS仪对多肽进行了产品性质认定和分析杂质性质，希望能建立一套完整的液质联用体系。

关键词：高效液相色谱-质谱，联用的条件，产品性质认定，杂质分析

Abstract

High-performance liquid chromatography – mass spectrometry (HPLC-MS) in conjunction with technology in recent years developed a new separation analysis techniques. It a very wide range of applications of separation methods – liquid chromatography with sensitive, proprietary, can provide information on molecular weight and structure of the combination of mass spectrometry has become an important separation of modern analysis techniques.HPLC-MS provide lots of valuable data for production and scientific research, and solve many previously difficult problems.This papermainly explores the LC-MS conditions, and the use of LC-ESI-MS instrument and analysis of impurities on the properties of the product to determine the nature of the polypeptide,hope to establish a complete set of liquid chromatography-mass spectrometry system.

Key Words：HPLC-MS, in combination with the condition, nature of the product identification, impurity analysis

摘要……………………………………………………………………………………………………………….. I

Abstract………………………………………………………………………………………………………….. II

第一章前言…………………………………………………………………………………………………. III

第二章实验部分……………………………………………………………………………………………. 6

2.1实验目的…………………………………………………………………………………………….. 6

2.2实验原理…………………………………………………………………………………………….. 6

2.3实验仪器与试剂………………………………………………………………………………….. 8

2.4 实验步骤……………………………………………………………………………………………. 8

2.4.1管道连接……………………………………………………………………………………. 8

2.4.2流动性制备………………………………………………………………………………… 8

2.4.3 一般参数配置……………………………………………………………………………. 8